Versatile Tools Towards Real-time, Single-molecule Biology
CIC nanoGUNE Seminars
- Speaker
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Aida Llauro, LUMICKS, The Netherlands
- When
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2019/05/08
13:00 - Place
- nanoGUNE seminar room, Tolosa Hiribidea 76, Donostia - San Sebastian
- Add to calendar
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Biological processes performed by proteins interacting with DNA/RNA, cell
membranes or cytoskeletal protofilaments are key to cell metabolism and life.
Detailed insights into these processes provide essential information for
understanding the molecular basis of life and the pathological conditions that
develop when such processes go awry. The next scientific breakthrough consists
in the actual, direct, real-time observations and measurements of the
individual mechanisms involved, in order to validate and complete the current
biological models. Single-molecule technologies offer an exciting opportunity
to meet these challenges and to study protein function and activity in real-
time and at the single-molecule level. Here, we present our efforts for
further enabling discoveries in the field of biology and biophysics using both
the combination of optical tweezers with correlative fluorescence microscopy
(widefield, TIRF, confocal and STED) and label-free Interference Reflection
Microscopy (IRM). We present several examples in which our technologies
enhanced the understanding of protein folding, cytoskeletal mechanics,
protein-membrane interactions and genome structure and organization.
Furthermore, we show that advances in hybrid single-molecule methods can be
turned into an easy-to-use and stable instrument that has the ability to open
up new venues in many research areas.
**Host** : R. Pérez-Jiménez