ONLINE - PhD Mid-term Seminar Series: Nano-Cinematography: Liquid-Phase TEM to study dynamics of nanoscale objects in native liquid sample environment
https://dipc.ehu.eus/en/scientific-activities/joint-seminar-agenda/cic-nanogune/seminar1393
https://dipc.ehu.eus/@@site-logo/dipc-logo.png
ONLINE - PhD Mid-term Seminar Series: Nano-Cinematography: Liquid-Phase TEM to study dynamics of nanoscale objects in native liquid sample environment
CIC nanoGUNE Seminars
- Speaker
-
Stefan Merkens, Electron Microscopy Group
- When
-
2020/06/08
13:00
- Place
- nanoGUNE online Webinar
- Add to calendar
-
iCal
Subscribe to Newsletter

****
**Nano-Cinematography: Liquid-Phase TEM to study dynamics of nanoscale
objects in native liquid sample environment**
Simultaneously visualizing (functional) nanoscale objects and triggering their
dynamics in native liquid sample environment has always been a crucial
interest in fields from (bio-) chemistry towards colloidal science. Liquid-
Phase Transmission Electron Microscopy (LP-TEM) is a new promising electron
microscopy imaging technique to study such nanoscale dynamics in native liquid
sample environment.1 Therefore, tiny amounts of sample solution are enclosed
between two ultrathin electron beam-transparent membranes forming a liquid
cell (LC) and which is integrated into fluidic TEM sample holders. However,
there is still one ultimate goal to be achieved in the context of chemistry,
which is to experimentally mix reagents, to describe a mechanism of chemical
reaction between them and to quantify its kinetics from time-resolved in situ
visual inspection at the nanoscale.
On the route to this goal, there are several obstacles to face, i.e.
accounting for radiolytic effects and their mitigation,2 tracking of fast
particle movement in liquid, extracting reaction kinetics from visual TEM
data, implementation of theoretical model description, understanding and
describing the hydrodynamics of solution replacement and reagent mixing inside
the very complex and confined space of fluidic LP-TEM system,3 etc.
In this talk, I will allocate state-of-the-art LP-TEM research in this
figurative obstacle race towards a reliable and quantitative technique. I will
illustrate how in situ particle imaging and tracking can provide new insight
into colloidal systems. I will further introduce a unique twofold experimental
and simulation-based approach to quantify the hydrodynamic properties of
complex micro/nanofluidic LP-TEM systems. Finally, I will demonstrate how our
work can support LP-TEM community in better planning of fluidic experiments
addressing the challenges mentioned above.
**References**
1. Ross, F. M. & de Jonge, N. Past, present and future electron microscopy of liquid specimens. 350, (2018).
2. Schneider, N. M. et al. Electron-Water interactions and implications for liquid cell electron microscopy. J. Phys. Chem. C 118, 22373–22382 (2014).
3. Ring, E. A. & De Jonge, N. Microfluidic system for transmission electron microscopy. Microsc. Microanal. 16, 622–629 (2010).
\----------
Please **REGISTER** for PhD Mid-term Seminar Series on Jun 8, 2020 11:00 AM
CEST at:
[https://attendee.gotowebinar.com/register/7273286317752548363](https://attendee.gotowebinar.com/register/7273286317752548363)
After registering, you will receive a confirmation email containing
information about joining the webinar.
Thank you very much for your participation!