ONLINE - PhD Mid-term Seminar Series: Humidity induces water nanolayers on emulated influenza surfaces: An AFM study
CIC nanoGUNE Seminars
- Speaker
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Maiara Iriarte, Self-assembly Group
- When
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2020/06/29
13:00 - Place
- nanoGUNE online Webinar
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** Humidity induces water nanolayers on emulated influenza surfaces: An AFM
study **
****
Maiara Iriarte
_Self-assembly Group, CIC nanoGUNE_
Seasonal epidemics of Influenza A virus cause annually 3 to 5 million cases of
severe flu illness and up to 650000 flu-related deaths, worldwide. These
seasonal outbreaks have been related to the environmental conditions,
specifically the relative humidity or temperature. Similar to SARS-CoV, both
survival and transmission of Influenza A virus are supported by cold and dry
climate conditions. While studies in ferrets and in guinea pigs have
demonstrated that cold temperatures and low relative humidity favour virus
survival and transmission, epidemiological evidence suggests that humidity
affects the virus transmission by modulating its survival. In contrast, there
is very limited research on the surface physics of the virus and,
specifically, on the surface glycoproteins. Studying how humidity affects the
Influenza A virus envelope structure is not only important to understand its
stability and survival outside the host cells, but also to design strategies
to interfere with transmission.
In this project, we have proposed to study the effect of humidity on the
Influenza A virus surface by atomic force microscopy (AFM) by two model
systems:
(i) Mannosyl-gold nanoparticles (Mann-Au NPs) as they can be produced in
similar size and glycan surface composition as the virus glycoproteins.
(ii) Model membranes, built from supported lipid bilayers (SLBs) and
incorporated surface glycoprotein hemagglutinin (HA).
Our findings on the model systems developed suggest that the Mann-Au NPs (i)
present a strong hydration ability, while results on the model membranes (ii)
indicate that HA protects the membrane damage after dehydration. These results
will contribute to understanding the (bio)physics of the virus surface in
humid air and to help to better control its transmission during seasonal
outbreaks.
**References**
1\. Petrova VN & Russell CA (2018) The evolution of seasonal influenza
viruses. Nat. Rev. Microbiol. 16, 47–60
2\. Otter JA, Donskey C, Yezli S, Douthwaite S, Goldenberg SD & Weber DJ
(2016) Transmission of SARS and MERS coronaviruses and influenza virus in
healthcare settings: the possible role of dry surface contamination. J. Hosp.
Infect. 92, 235-250
3\. Vejerano EP & Marr LC (2018) Physico-chemical characteristics of
evaporating respiratory fluid droplets. J. R. Soc. Interface 15,
doi:10.1098/rsif.2017.0939
4\. Chiantia S, Kahya N & Schwille P (2005) Dehydration Damage of Domain-
Exhibiting Supported Bilayers: An AFM Study on the Protective Effects of
Disaccharides and Other Stabilizing Substances. Langmuir 21, 6317-6323
5\. Barinov N, Ivanov N, Kopylov A, Klinov D, Zavyalova E (2018) Direct
visualization of the oligomeric state of hemagglutinins of influenza virus by
high-resolution atomic force microscopy. Biochimie 146, 148-155
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