Genealomics, single cell lineage tracing of blood cell production through next generation sequencing
CIC nanoGUNE Seminars
- Speaker
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Leila Perie, Institut Curie, Paris, France
- When
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2022/05/02
13:00 - Place
- nanoGUNE seminar room, Tolosa Hiribidea 76, Donostia - San Sebastian
- Add to calendar
-
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**Genealomics, single cell lineage tracing of blood cell production through
next generation sequencing**
Leila Perie, Institut Curie
How heterogeneous systems of cells constituting multicellular organisms
establish, organize and achieve coordination persists as a central question in
natural sciences. Whereas stochastic gene or protein expressions have clearly
demonstrated their role in cellular heterogeneity and are widely studied, the
role of cell heterogeneity in the organization of multicellular organisms has
been less interrogated. Addressing this question requires adequate tools that
quantitatively study ensembles of cells individually rather than group of
cells.
My research aims at addressing cell heterogeneity in dynamical and complex
systems of cells using the hematopoietic system as a model of study.
Strikingly hematopoietic cells (immune cells, platelets and red blood cells)
compose over 90% of total human cells and correspond to approximately ten
trillions of cells. More importantly they all originate from the same cells,
the hematopoietic stem cells (HSC), through a process called hematopoiesis. In
addition, as immune and blood cells have a short life span (from hours to
months) and can response to perturbations like infections, this process is
highly dynamical. It is therefore an interesting and challenging model to
study differentiation in a complex system at the single cell level.
Genealomics, which use high throughput sequencing methods to track the
descendant of individual cells, is a powerful tool to study differentiation at
the single cell level. In my research, we take advantage of genealomics
methods to study the hematopoietic differentiation pathway both in mice and
human. I will present some recent results using genealomics to study blood
cell production.
**Host:** J. M. Pitarke