Membrane mechanics and dynamics: From in-vitro to in-vivo

DIPC Seminars

Adai Colom Diego, Biofisika Institute, UPV/EHU, Bilbao
Online Seminar, Donostia International Physics Center
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Membrane mechanics and dynamics: From in-vitro to in-vivo Organelles and cells are delimited by a lipid bilayer, which is highly deformable, a property essential to many cell processes such as motility, endocytosis, cell division. During these deformations, lipid membranes experience stretch causing membrane tension. Membrane tension and membrane proteins are main regulators of the cell processes that remodel membranes, albeit, it is difficult to measure in vivo. FliptR (for Fluorescent LIPid Tension Reporter) can monitor changes of membrane tension by changing its fluorescence lifetime as a function of the twist between its fluorescent groups, allowing for an easy quantification of membrane tension by fluorescence lifetime imaging microscopy (FLIM). From other hand, HS-AFM has been proven a unique and powerful tool for the analysis of the structure and dynamics of single biomolecules which change the membrane properties as ESCRT III, Dynamin or antibiotic treatments. Host: David de Sancho ZOOM: YouTube: